首页> 外国专利> METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID, CARTRIDGE FOR SEPARATING AND PURIFYING NUCLEIC ACID AND KIT FOR SEPARATING AND PURIFYING NUCLEIC ACID

METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID, CARTRIDGE FOR SEPARATING AND PURIFYING NUCLEIC ACID AND KIT FOR SEPARATING AND PURIFYING NUCLEIC ACID

机译:分离和纯化核酸的方法,分离和纯化核酸的试剂盒和分离和纯化核酸的试剂盒

摘要

PPROBLEM TO BE SOLVED: To provide a method for separating and purifying nucleic acid, which has excellent separation performance and improved cleaning efficiency, is simple and rapid, has excellent automation and miniaturization suitability and in which a porous membrane having substantially the same separation performance and being mass produced is used. PSOLUTION: In the method for separating and purifying nucleic acid comprising (1) a process for passing a sample solution containing nucleic acid through a nucleic acid adsorbing porous membrane to adsorb nucleic acid on the porous membrane, (2) a process for cleaning the porous membrane on which nucleic acid is adsorbed and (3) a process for desorbing nucleic acid from the porous membrane by using a recovering liquid, the nucleic acid adsorbing porous membrane is a porous membrane on which nucleic acid is adsorbed by interaction substantially not associated with an ionic bond and a process for drying the porous membrane having adsorbed nucleic acid is not included between the cleaning process and the recovery process. PCOPYRIGHT: (C)2005,JPO&NCIPI
机译:

要解决的问题:提供一种分离和纯化核酸的方法,该方法具有优异的分离性能和提高的清洁效率,简单,快速,具有优异的自动化和小型化适用性,并且其中具有基本相同的多孔膜使用分离性能并大量生产。

解决方案:在分离和纯化核酸的方法中,包括(1)使含有核酸的样品溶液通过核酸吸附多孔膜以使核酸吸附在多孔膜上的过程,(2)用于分离核酸的方法。清洗其上吸附有核酸的多孔膜,以及(3)使用回收液从多孔膜上解吸核酸的方法,该核酸吸附多孔膜是基本上不通过相互作用而吸附核酸的多孔膜。在清洁过程和回收过程之间不包括与离子键相关的分子和干燥具有吸附的核酸的多孔膜的过程。

版权:(C)2005,JPO&NCIPI

著录项

  • 公开/公告号JP2005154416A

    专利类型

  • 公开/公告日2005-06-16

    原文格式PDF

  • 申请/专利权人 FUJI PHOTO FILM CO LTD;

    申请/专利号JP20040293641

  • 发明设计人 IWAKI YOSHIHIDE;

    申请日2004-10-06

  • 分类号C07H21/04;B01D69/12;B01D71/16;B01J20/02;B01J20/24;C07H21/00;C12N15/09;

  • 国家 JP

  • 入库时间 2022-08-21 22:36:45

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