METHOD FOR GENE DETECTION BASED ON HUMAN MITOCHONDRIA GENE VARIATION

摘要

PROBLEM TO BE SOLVED: To provide a method for detection, by which useful information for judging prediction of progress of blood vessel lesions being an important diabetic complication is provided by searching mitochondrial gene polymorphism related to centenarians and diabetic patients having blood vessel lesions.;SOLUTION: The whole base sequences of mitochondrial genomes of 96 centenarians and 96 diabetic patients having blood vessel lesions (at least one symptom of diabetic retinopathy, diabetic nephropathy, ischemic heart disease and arteriosclerotic vaso-occlusive) are analyzed by a direct base sequence determination method. As a result, the diabetic patients are confirmed to have variations of 512 parts in all. Among them, variations of 346 parts (243 synonymous substitutions and 103 un-synonymous substitutions) are confirmed in a coding region and variations of 170 parts are confirmed in a non-coding region, respectively. The progress of the blood vessel lesions is predicted by analyzing the variation parts in detail.;COPYRIGHT: (C)2005,JPO&NCIPI