METHOD FOR DETERMINING BASE TYPE, PRIMER AND KIT FOR DETERMINING BASE TYPE

摘要

PROBLEM TO BE SOLVED: To provide a method for determination of a required base type in a nucleic acid more accurately and more reproducibly and to provide a primer and kit for determining the same.;SOLUTION: The base type determination primer for determining the base type of a monobasic substituted region of a target nucleic acid, wherein the primer consists of a single-stranded nucleic acid which is capable of hybridizing to the target nucleic acid such that a 3' terminal thereof corresponds to the substituted region of the target nucleic acid, and wherein the single-stranded nucleic acid includes: a substitution corresponding region located at the 3' terminal and consisting of a base complementary to any one of predictable types of bases in the substituted region of the target nucleic acid; a noncomplementary region adjacent to the substitution corresponding region on the 5' terminal side thereof and consisting of two bases noncomplementary to the target nucleic acid; and a complementary region adjacent to the noncomplementary region on the 5' terminal side thereof and consisting of five or more bases complementary to the target nucleic acid.;COPYRIGHT: (C)2005,JPO&NCIPI