SPECIFIED GENE DETECTING METHOD, AND BASE-MODIFIED NANOPARTICLE AND REAGENT FOR DISCRIMINATING MONOBASIC VARIATION TYPE GENE USED THEREFOR

摘要

PPROBLEM TO BE SOLVED: To provide a specified gene detecting method capable of detecting quickly and easily a monobasic variation or the like in a gene, for example, and capable of identifying further a kind thereof. PSOLUTION: The specified gene is detected using a nanoparticle coupled with a base and having an electrochemical activity. In concrete saying, the monobasic-modified nanoparticle is made to act on the electrode immobilized with the gene of a discrimination object, and the presence of coupling of the nanoparticle to the electrode is detected as an electrochemical signal to discriminate the presence of the variation in the gene. Four kinds of nanoparticles of a cytosine-modified nanoparticle, a thymine-modified nanoparticle, a guanine-modified nanoparticle, and an adenine-modified nanoparticle are used as the monobasic-modified nanoparticles, and those are made to act sequentially to detect the electrochemical signals, so as to determine the kind of the variation in the gene. The each nanoparticle is a nanoparticle having the electrochemical activity, and is a gold nanoparticle, for example. PCOPYRIGHT: (C)2005,JPO&NCIPI