Methods of directing C-O bond formation utilizing a type II polyketide synthase system

摘要

The present invention provides methods of modifying a biological molecule by CO bond formation utilizing a type II polyketide synthase (PKS) system from the nonactin biosynthesis gene cluster. The type II PKS responsible for biosynthesis of the macrotetralide nonactin includes polypeptides encoded by the nonJK genes. The NonJ and NonK polypeptides have been identified by the inventors as ketoacyl synthases capable of directly catalyzing CO bond formation between substrate molecules. This invention increases the scope and diversity of chemical syntheses available for drug design and combinatorial biosynthesis.