The present invention relates generally to the field of generating genetically modified C57 mice. More particularly, the present invention pertains to 1) blastocyst-derived mouse embryonic stem cell (ES) cell lines including, but not limited to, the IC1, IC2, IAC1, IAC2, IAC3, IAC4, IAC5, IAC6, IAC7 or IAC8 ES cell line, 2) to efficient methods of making genetically modified C57 mice by introducing the modified C57 ES cells into the mouse blastocysts of either the same mouse strain and/or color of albino C57 strain, or other C57 strain, to generate genetically modified novel, useful and hereto unknown models of C57 mice, and to methods for identifying the chimerism of chimeras which can be not known by coat color.
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机译:本发明总体上涉及产生基因修饰的C57小鼠的领域。更具体地,本发明涉及1)囊胚来源的小鼠胚胎干细胞(ES)细胞系,包括但不限于IC1,IC2,IAC1,IAC2,IAC3,IAC4,IAC5,IAC6,IAC7或IAC8 ES 2)通过将经修饰的C57 ES细胞引入具有相同小鼠品系和/或白化病C57品系或其他C57品系颜色的小鼠胚泡中以产生经遗传修饰的新型,有用和迄今未知的C57小鼠模型,以及鉴定嵌合色的嵌合现象的方法,这些现象尚不能通过皮毛颜色得知。
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