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Enrichment of phosphate peptides for proteomic analysis
Enrichment of phosphate peptides for proteomic analysis
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机译:富集磷酸肽用于蛋白质组学分析
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摘要
The present invention is a method, and a kit for carrying out the method, of separating phosphorylated peptides from a mixture of phosphorylated and unphosphorylated peptides. The method involves reacting a collection of peptides, in which some of the peptides have one or more phosphate groups, with a first resin. The peptides are then reacted with a capture ligand which reacts with the phosphate group of the phosphorylated peptides to form a bond. The capture ligand is used to separate the phosphorylated peptides from the unphosphorylated peptides.
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