Analytical method of High Performance Liquid Chromatography to detect and quantify the lactone sesquiterpenica dehidroleucodina (DHL) in wet and Dry extracts of plant species

摘要

High Resolution chromatographic procedure to detect and accurately quantify dehidroleucodina in plant extracts, from both dry and fresh leaves. This procedure is sufficiently sensitive to detect DHL with a Detection limit of 1.7 ng / ml and a limit of quantification of 17 ng / ml in less than 8 minutes.These are the first results of the original determination of LDH in a Biological System.Claim 1: analytical method of High Performance Liquid Chromatography to detect and quantify the lactone sesquiterpu00e9nica dehidroleucodina (DHL) in wet and Dry extracts of plant species; characterized by the following steps: a) understanding is extr Action of plant species from the sample in acetone dried Aerial part, STEM and leaves.The Plant; (b) separated by columns using exchange resin, a 200 mm KH2PO4 Solution as Mobile phase in a isocru00e1tico mode at a flow rate of 0.55 ml / min; (c) to automatically Post column with o phthalaldehyde; and (d) are detected using 360 nm fluorescence Excitation and Emission at 450 nm.