Isolation of Penicillium sp. seeds (cotyledons) of coffee (Coffea arabica L.) variety 'new world', its cultivation and isolation of mycophenolic acid and a derivative of mycophenolic acid 7 - hydroxy - 5 - methoxy - 4 - metilfitalida of extract.

摘要

"Isolation of Penicillium sp. seeds (cotyledons) of coffee (Coffea arabica L.") Variety 'new world' the cultivation and isolation of mycophenolic acid and a derivative of mycophenolic acid 7 - hydroxy - 5 - methoxy - 4 - metilfitalida of extract.This is a project that aimed to cooperate with the development of methodologies for the analysis of the constituents of the drink of coffee.Coffee is a culture of great economic interest because it generates billions of jobs and moves billions of dollars around the world.Historically, Brazil occupies the position of world's leading producer and exporter of this product, but in recent years has lost the market, depending on the quality of coffee it produces.In front of the market increasingly demanding quality. The healthy tissues (fruit and seed benefited) of coffee variety 'new world' (Coffea arabica L.) and Fruit Coffee "icatu" a hybrid (Coffea arabica L. x Coffea canephora P.) were obtained approximately 64 isolates of fungi endofu00edticos, thus underlining, a symbiotic relationship between the microorganisms and coffee. Among the fungi isolates of C. Arabica L.The fungus Penicillium sp. was grown in liquid medium and solid medium, Czapek's, in which it was used coffee (ground) as a substrate.This was further subjected to tests of biotransformation in the presence of caffeine, acid felu00farico, quinico, cinnamic acid and 3,4 - metilenodioxicinu00e2mico.These experiments led to the isolation of 7 - hydroxy - 5 - methoxy - 4 - metilfitalida, which smells like coffee.Here probably have occurred a transposition of genes between the coffee and the fungus (two different realms), which would be a real genetic engineering in vivo and.This substance is described for the first time in a cultivation of fungi and of mycophenolic acid, a substance known, which exhibits immunosuppressive activity.