Method for monitoring of prostate cancer and its treatment, combined use of molecular techniques and biomarkers of androgen receptor (AR).Human glandular calicreu00ecna 1 (klk2) and gene DD3, oligonucleotu00ecdeos and use

摘要

Method for monitoring of prostate cancer and its treatment, combined use of molecular techniques and biomarkers of androgen receptor (AR).Human glandular calicreu00ecna 1 (klk2) and gene DD3, oligonucleotu00ecdeos and use ".The invention provides methods for monitoring of patients with prostate cancer by combined use of molecular biology techniques.Specifically, the technology of RT-PCR semi quantitative multiplex, components and reaction conditions to estimate the relative abundance of specimens of RNAs of genes air.Klk2 and DD3 differentially expressed in prostatic tissue of patients with adenocarcinomas, comparing them with benign prostatic diseases (hyperplasia, prostatic infections among others).The invention also provides a method that determines a predictive index of the disease, for the three genes for patients suspected of prostate cancer based on expression of the air.Klk2 and DD3 in biopsies of prostatic tissue.This invention also provides methods for the monitoring of patients with prostate cancer by combined use of molecular biology techniques, specifically the technology RT PCR nested.Oligonucleotides, chemical components and reaction conditions to detect specimens of RNAs of genes klk2 and DD3, differentially expressed in peripheral blood.To monitor the presence or absence of micrometastu00e1ticas cells circulating in the peripheral blood of patients with prostate cancer.The present invention has direct application in the distinction between patients with prostate cancer and those with benign hyperplasia, as well as the choice of the most appropriate therapy.According to the staging of the disease. In addition, it helps monitoring the therapeutic efficacy and relapse of disease after treatment.