Process for the production of the enzyme recombinant human copper zinc superoxide dismutase (rhSOD) enzymes superoxide dismutase (SOD) or proteins

摘要

Process for the production of recombinant human enzyme copper zinc superoxide dismutase (rhSOD) enzymes superoxide dismutase (SOD), or proteins.In this case, belonging to the biotechnology sector, refers to a process for the production of the enzyme recombinant human copper zinc superoxide dismutase citopiasmu00e1tica or extracellular,As indicated by rhSOD, or other enzymes superoxide dismutase, as enzymes manganese superoxide dismutase, iron superoxide dismutase and other enzymes superoxide dismutase, natural or recombiantes,With intra - or extracellular expression, as indicated by SOD, and proteins.That includes the steps of aerobic or anaerobic cultivation of microorganisms with the production of rhSOD or sod or protein during the cell growth; the breakup, disintegration.Lysis or cell permeabilization for the separation of the enzyme or intracellular protein; and, after its release to the liquid phase, the separation of the enzyme or protein.To obtain a product more easily purificu00e1vel,The proposed process includes alternatives to chemical and biological cell permeabilization for the separation of recombinant enzyme in the interior of the microorganism that produces it.We then can be broken by mechanical method or by a combination of different sequ00b3u00eancias of these three methods.In order to achieve the best performance of the process of separation of this enzyme in its production.The novelty and inventive activity of this process include the option of implementing process in batch and in obtaining successive lysis.Disintegration or permeabilization of the cell wall of recombinant microorganism, using surfactants.As the detergent lauryl ether sodium sulfate and sodium lauryl sulfate on chemical methods and enzymes 225 - 1,3 glucanase purified or not on biological methods for the recovery of rhSOD,Sod or proteins.