DNA Sequence encoding a protein cryiii modified for Greater toxicity against Coleoptera; Proteins cryiii modified method to improve the toxicity of a protein cryiii; ProcessTo combat the coleptero of Colorado potato (L

摘要

New modified Cry Proteins, in particular Proteins cryiiia modified, which presents a significantly altered Toxicity and DNA sequences coding for the same.Through the analysis by alanine Scanning mutagenesis allowed us to identify individual amino acids involved in the toxicity against Corn Rootworm.Random replacement of these amino acids can identify Proteins modified with increased toxicity, the combination of all or most of these amino acids so identified in a SunProtein allows cryiiia modified Proteins with a significantly higher toxicity.Idetificaron is, in particular, the protruding regions of the Molecule Cry and is located at the apex of The Cry Proteins, such as former regions in Toxicity.