CLONING VECTOR WITH A BLUNT-END INSERT FOR USE IN POSITIVE SELECTION OF RECOMBINANT CLONES, CLONING METHOD FOR THE SAID VECTOR, USE OF THE SAID VECTOR FOR THE EXPRESSION OF AN INSERT

摘要

cloning vector comprising a dna sequencecoding for a protein and an enzyme poison sitelocated in a restricted core coding regionthe nucleotide sequence of the enzyme.restriction of free ends and being active within terms of the ligature. a method of selectionrecombinant clones. and the use of a vector to thethe selection of recombinant clones and cloning, thethe sequencing or amplification of an insert of interest.