SCREENING METHOD FOR SCREENING ACTIVE COMPOUNDS OR NATURAL EXTRACTS HAVING ACTIVATORY EFFECTS ON 5' AMP-ACTIVATED PROTEIN KINASE

摘要

The present invention provides an efficient screening system for searching for a substance which increases the variety of cell or tissue 5 'AMP- activated protein kinases (5' AMP-activated protein kinase, AMPK) activity in the. Conventional screening or assay method is measured by using the isotope to, many samples as well as the need to adopt a complicated step as required, and the AMPK in many levels in order to find the activated AMPK in the process of screening is treated under the same conditions which together with the time constraints facing the drawback of doubling the risk. I In contrast, the screening model for ELISA method based on the immunoassay of the present invention is the analysis of many types of samples at a time is possible, of course, provides a method capable of effectively measuring in a sample the concentration of a small amount. Through the method of the present invention a compound library is, of course, native plants, not only can effectively effectively screen the AMPK activity promoting substances from natural products library of medicinal herbs such as, containing the screening material, as an active ingredient, obesity prevention and treatment, Diabetes It can be used in the development of prevention and treatment, prevention and treatment, cancer prevention and treatment of ischemic disease, and hypoglycemic agents or herbal remedies, as well as for strengthening, and food materials that are effective against fatigue.