Isolation and culture-expansion methods of mesenchymal stem/progenitor cells from umbilical cord blood, and differentiation method of umbilical cord blood-derived mesenchymal stem/progenitor cells into various mesenchymal tissues

摘要

The present invention relates to a method inducing the differentiation of the separation method and cultured mesenchymal tissue of the bulb cord blood-derived mesenchymal stem cells and cells.; The cells isolated culture method of the present invention Ficoll cord blood-then superimposed on hayipaek solution, centrifugation mononuclear obtain a layer of cells precipitate, cells of mesenchymal stem cells obtained by monolayer culture of the mononuclear cells and progenitor cells antibodies to the specific antigen and a period of time reaction, using a cell separator by culturing separate only the antibodies and combination of cells, thereby obtaining a high purity and excellent viability mesenchymal stem cells and progenitor cells, with the FIG.; Mesenchymal stem cells and progenitor cells of the present invention have the ability to differentiate into the various mesenchymal tissues, including chondrocytes, or bone cell differentiation medium and under suitable conditions.; Thus, through the cell isolation culture method of the present invention can produce large quantities of stem cells and mesenchymal progenitor cells, the resulting cells can be useful in the treatment and regeneration of injured mesenchymal tissues.