BACILLUS LICHENIFORMIS MUTANT GN-M10 AND PRODUCTION METHOD OF PROTEASE WITH IT

摘要

PURPOSE: A microorganism Bacillus licheniformis mutant GN-M10 and a production method of protease using the same microorganism are provided, which mutant microorganism has improved protease-producing activity, so that the protease can be mass-produced. CONSTITUTION: The microorganism Bacillus licheniformis mutant GN-M10(KCTC 10417BP) is provided, wherein Bacillus licheniformis mutant GN-M10(KCTC 10417BP) is obtained by collecting a microorganism GN-55 having high protease activity from the soil, and treating the microorganism GN-55 with NTG. The method for producing protease comprises culturing Bacillus licheniformis mutant GN-M10(KCTC 10417BP) in a medium and recovering protease from the cultured medium, wherein the medium contains 3 to 5% of soluble starch as a carbon source and 2 to 3% of cotton seed protein as an organic nitrogen source.