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ISOLATION METHOD OF SECONDARY PRODUCT HAVING WEEDING EFFICACY AGAINST WHITE CLOVER RELEASED FROM CULTURE FLOID OF SCLEROTINIA TRIFOLIORUM BWC98-105
ISOLATION METHOD OF SECONDARY PRODUCT HAVING WEEDING EFFICACY AGAINST WHITE CLOVER RELEASED FROM CULTURE FLOID OF SCLEROTINIA TRIFOLIORUM BWC98-105
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机译:次生产品具有抗白三叶草菌BWC98-105培养液释放白三叶草功效的分离方法
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摘要
PURPOSE: Provided is an isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens), which is released from culture extract of isolate BWC98-105(Sclerotinia sp.), thereby withering the subterranean stem and stem of the white clover continuously, and inhibiting the generation thereof consequently to reduce the consumption of agricultural chemicals. CONSTITUTION: An isolation method of a secondary metabolic product with herbicidal effect on the white clover(Trifolium repens) by using BWC98-105(Sclerotinia sp.) comprises the steps of: culturing BWC98-105 strain in PD broth medium for 7 days, followed by systematic extraction with an organic solvent; fractioning the extraction with butanol and ethylacetate; separating the butanol and ethylacetate fractions using the column filled with reverse phase silica gel(C18); and analyzing the separated fractions by HPLC, wherein a second metabolic product having herbicidal effect on the white clover(Trifolium repens) is produced at retention time intervals of 4.13 minute.
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