RAFFINOSE SYNTHASE GENE, PROBE AND PRIMER, METHOD FOR DETECTING AND METHOD FOR AMPLIFICATION OF NUCLEIC ACID COMPRISING RAFFINOSE SYNTHASE GENE, METHOD FOR PREPARING RAFFINOSE SYNTHASE GENE, EXPRESSING VECTOR, RAFFINOSE SYNTHASE

摘要

FIELD: biotechnology, biochemistry, molecular biology.;SUBSTANCE: raffinose synthase is prepared by culturing the host-cell transformed with vector comprising raffinose synthase gene. Prepared raffinose synthase is isolated from cultural liquid. Partial nucleotide sequence of gene encoding raffinose synthase is used as a probe or primer. Invention allows carrying out the modulation of level of expression and activity in plants.;EFFECT: improved preparing method, valuable properties of gene and enzyme.;24 cl, 8 ex