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Thermostable firefly luciferase, thermostable firefly luciferase gene, manufacturing method of a novel recombinant DNA and heat resistance firefly luciferase
Thermostable firefly luciferase, thermostable firefly luciferase gene, manufacturing method of a novel recombinant DNA and heat resistance firefly luciferase
PROBLEM TO BE SOLVED: To provide a method for producing a heat-resistant firefly luciferase by changing specific amino acid residues in a mild type firefly luciferase into hydrophobic amino acid residues.;SOLUTION: This heat-resistant firefly luciferase is characterized by changing the amino acids at the 217- and 315-positions in the amino acid sequence of the mild type firefly luciferase or the amino acids at the positions equivalent to the 217- and 315-positions in the amino acid sequence of Luciola cruciata or Luciola lateralis luciferase into hydrophobic acid residues. The heat-resistant firefly luciferase gene. A vector integrated with the gene. The method for producing the heat-resistant firefly luciferase comprises using the vector to produce the heat-resistant firefly luciferase. The heat-resistant firefly luciferase can efficiently be obtained by the method, and is highly useful on industry.;COPYRIGHT: (C)2005,JPO&NCIPI
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