Separated method of sugar in glycoside connection content chemical compound, sugar separated system, reagent kit for sugar separation, standardization sample, and appraisal system for sugar separation

摘要

The inflow chemical reaction system of this invention (A), from the sample fill device (4), the reaction tube where the alkaline solution (1) flows continually (7) introduces the sample solution which includes the glycoprotein. When the sample solution and the alkaline solution (2) the mixed solution reaches in the constant temperature oven (6), the cutting reaction of glycoside connection advances, the sugar chain is separated from the glycoprotein. The alkali in the solution which includes this sugar chain is removed the ion exchange column (12) by, the sugar chain (14) the sample tube (13) to collect it is possible only fraction. In the inflow chemical reaction system (A) the reaction time is the amount unit, former method compared to reaction time is shortened substantially. Because of this, quick and the simplicity, at the same time it cuts off glycoside connection in high accuracy, it is possible to separate the sugar from glycoside connection content chemical compound.