Measurement and calibration method for sample injection volume and mobile phase delivery rate in a ultra micro-scale liquid phase delivery system

摘要

The present invention provides a method for measuring and calibrating sample injection volume or mobile phase delivery rate of any type of micropumps or their integrated systems by using the direct proportion of the total activity (or mass) to the delivery rate (or volume) of a radiochemical nuclide with known activity in a certain time period. Also, the present invention may adjust the range of measurement and calibration from micro-liter (μL) to pico-liter (pL) or from μL/min to pL/min by selecting nuclide species and their concentrations from different liquid calibration radiation sources.