Integrated knowledge-based reverse engineering of metabolic pathways

摘要

A method of enhancing the yield of a product is provided. A host organism that is adapted to produce the product as a function of metabolism in the presence of a substrate is selected and a plurality of optimal reaction pathways are determined by searching a database and executing an optimization algorithm. The optimal reaction pathways take place in the host organism to produce the product from the substrate and are ranked and enumerated. The optimization algorithm comprises a flux balance analysis and maximizes the yield of the product.