Stable strains of xylose and arabinose fermenting zymomonas mobilis, transposon for insertion, stable of alien genes within a bacterial genome, plasmid vector containing said transposon, bacteria transformed with said plasmid, vector pzb1862-idhl-ara atcc with ascension number pta-1798, transposable element mini-tn5-tal / tkt-xilaxilb atcc with ascension number pta-1795, bacterium strain g8 atcc with ascension number pta-1796, bacterium strain c25 atcc with ascension number pta-1799, ax atcc bacterial strain with ascension number pta-1797, reaction mixture comprising fermentable medium

摘要

"ZYMYMES MOBILIS FERMENTING ZERO AND ARABINOSIS STORAGE CODES, TRANSPOSON FOR STABLE INSERTION OF ALIEN GENES WITHIN A BACTERIAL GENOME, PLASMID VECTOR CONTAINING THE TRANSPOSMED-PERMED BETTER TRANSFORMED WITH THE TRANSFORMED-PERMITORED PLASTER PTA-1798, TRANSPONABLE ELEMENT MINI-TN5-TAL / TKT-AXILAXILB ATCC WITH ASCENTION NUMBER PTA-1795, G8 ATCC BACTERIA CUP WITH PTA-1796 ASCENTION NUMBER, C25 ATC-C99 BUTTER CUP WITH N99 AX ATCC BACTERIA CEPA WITH RANGE NUMBER PTA-1797, REACTION MIX UNDERSTANDING A FERTILIZABLE MEDIA ". The present invention briefly includes a transposon for the stable insertion of foreign genes within a bacterial genome, comprising at least one operon having gene structures encoding enzymes selected from the group consisting of xylAxilB, araBAD and tal / tkt, and at least one. promoter for the expression of gene structures in the bacterium, a pair of inverted insertion sequences, with operons contained within the insertion sequences, and a transposase gene located on the side of the insertion sequences. Also provided is a plasmid launcher vector for transforming foreign genes into a bacterial genome, comprising at least one operon having gene structures encoding enzymes selected from the group consisting of xylAxilB, araBAD and tal / tkt, at least one promoter for expression. of the gene structures in the bacterium, and at least two DNA fragments showing homology to a gene in the genome of the bacterium to be transformed. The transposon and the launcher vector are useful for constructing significantly different Zymomonas mobilis strains according to the present invention which are useful for converting cellulose derived pentose sugars into fuels and chemicals using traditional fermentation technology because they are stable for expression in a non-selective medium.