INHIBITOR AND ANTI-INHIBITOR MONOCLONAL ANTIBODIES SPECIFIC FOR HORSERADISH PEROXIDASE

摘要

Monoclonal antibodies have been preparedwhich are of the IgG isotype and are highly specificfor horseradish peroxidase. One group of antibodiesinhibits at least about 95% of the normal activity ofhorseradish peroxidase when bound to the enzyme. Asecond group of antibodies inhibits less than about20% of the enzymatic activity when bound to theenzyme, but prevents the binding of the antibodiesfrom the first group. The antibodies in either groupcan be conjugated to specific binding ligands such asdrugs or hormones.