PRESERVATION METHOD AND TRANSPORT METHOD FOR BLOOD FOR LEUCOCYTE INCUBATION, PRESERVATION METHOD AND TRANSPORT METHOD FOR PERIPHERAL BLOOD MONOCYTES AND METHOD OF INCUBATING LEUCOCYTES USING THE SAME

摘要

It is intended to provide a method whereby leucocytes can be easily separated from the blood collected from a patient and preserved and/or transported while sustaining a high proliferation ability, thereby achieving excellent effects as preservation and/or transport methods for the blood for leucocyte incubation to be used in immunological cell therapy. It is also intended to provide an incubation method with the use of leucocytes preserved and/or transported by the above methods. Namely, the blood for leucocyte incubation is preserved at 16 to 22oC so that peripheral blood monocytes can be well separated 10 hours, in particular, 30 hours after the blood collection and a high proliferation ability can be sustained. In the case where peripheral blood monocytes are separated before the preservation, the temperature range suitable for the preservation and/or transport is from 5 to 22oC.