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METHOD FOR IDENTIFYING NOVEL GENES INVOLVED IN THE REGULATION OF ANGIOGENESIS, STUDY OF SAID GENES AND USE THEREOF FOR THERAPEUTIC PURPOSES
METHOD FOR IDENTIFYING NOVEL GENES INVOLVED IN THE REGULATION OF ANGIOGENESIS, STUDY OF SAID GENES AND USE THEREOF FOR THERAPEUTIC PURPOSES
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机译:确定涉及血管生成的新基因的方法,有效基因的研究及其在治疗中的用途
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摘要
A process for identifying genes coding for cellular constituents involved in regulating angiogenesis including culturing endothelial cells on an extracellular matrix protein according to at least four different types of conditions: a reference condition, an angiogenesis promoting condition, an angiogenesis inhibiting condition, and a control condition; isolating messenger RNAs stemming from cells cultured according to the different conditions; and comparing at the qualitative and/or quantitative level, different messenger RNA populations to identify messenger RNAs stemming exclusively or in a particularly elevated quantity from cell cultures under conditions stimulating and/or inhibiting angiogenesis, the messenger RNAs corresponding to the genes coding for the cellular constituents involved in regulating angiogenesis.
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