Method for genotyping of single nucleotide polymorphism by allele-specific polymerase chain reaction using an internal positive control and a pair of primers, both of which are allele-specific

摘要

The present invention is a single by being through the allelic gene-specific PCR on the method of analyzing a single nucleotide polymorphism, specifically performing allele-specific PCR using the primer pair including the nucleotide sequence of the allelic gene polymorphic site a method for analyzing a nucleotide polymorphism, if more used as an internal positive control primer pair, and a SNP present in the adjacent position, characterized in that the allele-specific primers containing each SNP site base adjacent positions by performing allele-specific PCR and to, to a method for analyzing polymorphism of the alleles. Analysis method of the present invention is easy to analyze the genotype of a single nucleotide polymorphism it is frequently occurring crops because more accurate and efficient than the existing methods of analysis, one allele gene-specific primers prepared in the present invention are nucleotide polymorphism It can be useful as a marker for analysis.