Recombinant plasmid PCI-NEO-ODC-NSP1 (OPTIONS PCI-NEO-ODC-E2-L, PCI-NEO-ODC-E2-M), containing the gene equivalent PROTEIN ornithine decarboxylase gene equivalent of nonstructural protein gene (equivalent fragment of the block protein) Virus (ON THE EXAMPLE OF VIRUS VEE), intended for development of means of preventing viral infectious diseases

摘要

u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u0430 pCI - neo ODC - nsP1 (options pCI - neo ODC - e2 - l, pCI - neo ODC - e2 - m), u043fu043eu043bu0443u0447u0435u043du043du0430u00a0 in august 2002 on the basis of the vector pCI - neo (Promega), u0434u0435u043fu043eu043du0438u0440u043eu0432u0430u043du043du0430u00a0 in specialized. a recombinant plasmids virological research institute of microbiology center of ministry of defence of the russian federation for u211602 / 1 (02 / 2,02 / 3) and the development of new u043fu043eu043au043eu043bu0435u043du0438u00a0 u043fu0440u0435u0434u043du0430u0437u043du0430u0447u0435u043du043du0430u00a0 u0434u043bu00a0 protective medicines against viral infectious diseases, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u0435u0442u0441u00a0 the following constructive features: the size of 7321 (options 7294, 7291) base pairs (sukhoy.); marker sign: resistance to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443; the size of the vector part: 5472 pair basis; structural elements of the vector forces website, u0444u0430u0433u0430 t7 promoter, forces pBR322, mr. and resistance to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443, multiple site u043au043bu043eu043du0438u0440u043eu0432u0430u043du0438u00a0, enhancer - promoter cmv and poly (a) sequence of the virus SV40,marker gene for resistance to u0430u043du0442u0438u0431u0438u043eu0442u0438u043au0443 g 418 under u043fu0440u043eu043cu043eu0442u043eu0440u043eu043c and u044du043du0445u0430u043du0441u0435u0440u043eu043c virus SV40,containing the 3 '- end of synthetic poly (a) sequence; description of cloned fragments of the genome of the virus: u043au0434u043du043a val (48 497) (nsP1) and u043au0434u043du043a gene u043eu0440u043du0438u0442u0438u043d carboxy lyases (ODC); u043au0434u043du043a genome fragment of val (8564 - 8986) (e2 - l), and u043au0434u043du043a gene fragment of the genome of the virus ODC; u043au0434u043du043a val (8990 - 9409 (e2 - m) and u043au0434u043du043a gene fragments ODC; size: 450 sukhoy.- the u043au0434u043du043a gene viral protein nsP1, sukhoy (423). - u043au0434u043du043a virus protein e2 gene fragment of the sukhoy, 420. - u043au0434u043du043a gene fragment of viral protein e2 - m); the site u043au043bu043eu043du0438u0440u043eu0432u0430u043du0438u00a0: ODC sites NheI - SaI 1; u043au0434u043du043a gene fragments of val BstXI site located between two pest sites gene ODC equivalents.