'T-12' VIRAL STRAIN OF ADENOVIRAL HEPATITIS WITH INSERTIONS - HEN'S HYDROPERICARDITIS (ADVHH) FOR MANUFACTURING DIAGNOSTIC KITS AND VACCINES AND EXPERIMENTAL STUDYING THE DISEASE

摘要

FIELD: medicine.;SUBSTANCE: the suggested strain refers to DNA-containing Adenoviridae type being of cubic type of symmetry, has no supercapsid membrane, virions sizes - 70-80 nm, shape - icosahedral. Moreover, it is resistant to the temperature of 56°C for 30 min, not less, and to the action of both ether and chloroform. It is inactivated with formalin, theotropin, dimer ethylenimine. The strain is cultivated in hepatic cell culture of hen's embryos, about 72-96 h after infection it causes characteristic foci of granulation (cells' rounding), the formation of separate rounded cells and foci of cell degeneration. Viral titer is within 10^5.5 TCD₅₀/ml. The strain enables to reproduce different forms of the disease mentioned for experimental investigations, it is highly useful for manufacturing certain diagnostic kits and vaccines, moreover, being of high antigenic and immunogenic activity.;EFFECT: higher efficiency.;2 ex