SOLUTION FOR LYOPHILIZING MAMMAL-DERIVED CELL OR SPERM

摘要

PROBLEM TO BE SOLVED: To provide such a solution for lyophilizing mammal-derived cells or sperms securing a some extent of time from sampling sperms to lyophilize these sperms when the sperms are to be lyophilized, and meet exceeding 80% blastocyst development percentage after fertilization using the thus lyophilized sperms.;SOLUTION: The solution for lyophilizing mammal-derived cells or sperms is provided, being obtained, based on the result of examining an EGTA[ethylene glycol bis(2-aminoethyl ether)tetraacetic acid] tris-hydrochloric acid buffer solution(50 mM NaCl, 50 mM EGTA, 10 mM Tris-HCl)(Non-Patent Literature 2), by increasing the concentration of the buffer solution(Tris-HCl) and either adding no osmotic pressure adjuster(NaCl) or decreasing its concentration. That is, this solution for lyophilizing mammal-derived cells or sperms comprises 10-100 mM of an anti-coagulant, 50-500 mM of a buffering agent and 0-10 mM of an osmotic pressure adjuster.;COPYRIGHT: (C)2008,JPO&INPIT