D-arosu is recovered from the enzyme reaction product which in application null to fractionation and the

摘要

PROBLEM TO BE SOLVED: To efficiently separate and collect D-amylose; and to provide a technically workable continuous production method concerning the production of the high-purity D-amylose.;SOLUTION: The method for separating and collecting the high-purity D-amylose comprises crystallizing the D-amylose by utilizing the property of slight solubility of the D-amylose in alcohol, and separating the crystal when collecting the D-amylose from an enzymic reaction product obtained by converting a part of a substrate convertible to the D-amylose to the D-amylose. The enzymic reaction product is obtained by passing a fed solution containing the substrate convertible to the D-amylose through a D-amylose-producing immobilized enzyme and/or a bioreactor using immobilized microorganisms. Various kinds of buffers, and the like, usually contained in the enzymic reaction product are not required to be removed when subjecting the reaction product to the action of the alcohol. The substrate convertible to the D-amylose is D-psicose, and the enzymic reaction product is a mixed solution of the D-psicose and the D-amylose. The mixed solution contains 35% D-psicose and 15% D-amylose.;COPYRIGHT: (C)2005,JPO&NCIPI