MODIFIED SINGLE-STRANDED DNA-BINDING PROTEIN DERIVED FROM EXTREMELY THERMOPHILIC BACTERIUM, AND METHOD FOR ISOTHERMALLY AMPLIFYING NUCLEIC ACID BY USING THE PROTEIN

摘要

PPROBLEM TO BE SOLVED: To establish a technique by which nonspecific amplification is controlled in the nucleic acid amplification in an isothermal amplification reaction to improve amplification accuracy. PSOLUTION: The modified single-stranded DNA-binding protein derived from an extremely thermophilic bacterium has an amino acid sequence exhibiting a function contributing to the improvement of amplification efficiency of a template nucleic acid in an isothermal amplification system using a chain-substituting polymerase. The protein has a modified site at which at least one of modification of deletion, substitution, addition and insertion of one or more amino acids is caused. PCOPYRIGHT: (C)2007,JPO&INPIT