Malachite green derivatives for immunoassay reagents to detect malachite green

摘要

Compounds and procedures are provided for use in the preparation of reagents that can be used in immunoassays for Malachite Green, Leucomalachite Green and Carbinol. The compounds are Malachite Green Chromatic derivatives conjugated to several positions of the Malachite Green Chromatic structure, which in turn are conjugated to antigenic polypeptides for formation of antibodies for use in immunoassays. The antibodies produced, when employed in immunoassays with specified procedures, are found to be able to detect Malachite Green Chromatic as determined by positive results with fish tissue and water sample containing Malachite Green Chromatic that has been detected by HPLC or LC-MS.