Multiplexed PCR assay for detection of bovine spongiform encephalopathy (BSE)-associated bovine prion protein gene (PRNP) polymorphisms

摘要

A multiplexed PCR assay system and method are provided for detection of bovine prion protein gene (PRNP) insertion/deletion polymorphisms that are associated with bovine spongiform encephalopathy (BSE), thereby identifying a population of cattle or individual therefrom that possesses an elevated level of putative resistance or susceptibility to BSE. Such system or method uses a mixture of primers targeting PRNP insertion/deletion polymorphisms in the putative promoter, intron 1, and 3′ untranslated region of the bovine PRNP gene. Also provided is a kit for identifying a population of cattle or individual therefrom that possesses an elevated level of putative resistance or susceptibility to BSE.