首页> 外国专利> Method and apparatus for selecting the desired cells or parts of cells, distribution device of a sperm cell.Method for selecting a desired viable sperm cell suitable for in vivo or in vitro and viable sperm cell

Method and apparatus for selecting the desired cells or parts of cells, distribution device of a sperm cell.Method for selecting a desired viable sperm cell suitable for in vivo or in vitro and viable sperm cell

机译:选择所需细胞或细胞部分的方法和设备,精子细胞的分布装置。选择适合体内或体外所需活细胞的方法和活精子细胞

摘要

Method and apparatus for selecting the desired cells or parts of cells, distribution device of a sperm cell.Method for selecting a desired viable sperm cell suitable for in vivo or in vitro and viable sperm cell of the invention refers to a method that uses the orientation of aA sperm cell to determine the differences of the cell due to the size, mass, or density.The differences of cells, such as differences in the mass of DNA, are used to distinguish the sperm cells that contain X chromosome of sperm cell containing Y chromosome.Therefore, have use in procedures of in vitro and in vivo. The orientation of individual sperm cells is determined by measuring the fluorescent light.The method uses a detector to measure the magnitude of fluorescence (for the measurement of DNA (sex) of the flat surface of the spermatozoon).And a second detector to measure the magnitude of the fluorescent light petrographic derived from a light source separately.The light source is derived from part of a separate optical system by phase contrast or dark field to provide the data for guidance.It is important to note that all the excitement and the fluorescent light is excluded from the second detection system for optical filters of the passage.Thereby obtaining a clearer signal of the edge cu00f3ncava (no sign of the fluorescence of the flat surfaces of the spermatozoon).It is reported that the orientation of a sperm cell.When determined by the passage of light by using the optical sistma phase contrast or dark field through a sperm cell of interest, provides greater efficiencies.Higher processing speeds and greater reliability in the results obtained.An additional aspect of the invention describes a laser immobilization or ablative for restraining or destroy unwanted sperm.
机译:选择所需细胞或细胞部分的方法和设备,精子细胞的分布装置。选择适合于体内或体外的所需活细胞的方法和本发明的活精细胞是指使用定向的方法。精子细胞的大小,以确定其大小,质量或密度的差异。细胞的差异(例如DNA的质量差异)用于区分包含X染色体的精子细胞。 Y染色体。因此,已在体外和体内程序中使用。单个精子细胞的方向是通过测量荧光来确定的,该方法使用检测器测量荧光强度(用于测量精子平面的DNA(性)的测量),然后使用第二个检测器测量荧光的强度。荧光岩谱的大小分别来自一个光源。该光源是通过相衬或暗场从一个单独的光学系统的一部分中得到的,以提供指导数据。重要的是要注意所有的激发和荧光第二个检测系统排除了该通道的光学滤光片,从而获得了更清晰的边缘c u00f3ncava信号(没有精子平面荧光的迹象),据报道精子的方向当通过使用光学的雌蕊相衬或暗场穿过感兴趣的精子细胞来确定光通过时,可以提供更高的效率。本发明的另一方面描述了用于抑制或破坏不需要的精子的激光固定或烧蚀剂。

著录项

  • 公开/公告号BRPI0506656A

    专利类型

  • 公开/公告日2007-05-15

    原文格式PDF

  • 申请/专利权人 SELECT XY LIMITED;

    申请/专利号BR2005PI06656

  • 发明设计人 ANDREW FRONTIN-ROLLET;

    申请日2005-01-19

  • 分类号A61B10;C12N5;C12N5/076;G01N15;G01N15/14;G01N21/41;G01N21/64;G01N33/483;G01N33/50;

  • 国家 BR

  • 入库时间 2022-08-21 20:59:19

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