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A METHOD FOR BRINGING ABOUT REFOLDING OF A PROTEIN
A METHOD FOR BRINGING ABOUT REFOLDING OF A PROTEIN
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机译:一种有关蛋白质重铸的方法
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摘要
: Proteins may be made by genetically engineered microorganisms, the protein being stored in the form of inclusion bodies (IB). The proteins in the inclusion bodies are in an insoluble and inactive form. They may be dissolved using a solubilisation reagent (18), and the resulting solution diluted so that the proteins refold into the active form. This refolding of the protein is enhanced by subjecting a solution or suspension of the protein to low intensity sound waves (25), at a low enough intensity that the protein is not denatured. The intensity may be between 10 and 100 mW/cm2.
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