A vector for the manufacture of IL-4 and IL-4 muteins in a strain of Escherichia coli, a vector comprising DNA sequence, Escherichia coli transformed with one or more vectors and use of the vector

摘要

A vector for the manufacture of IL-4 and IL-4 muteins in a strain of Escherichia coli, comprising in 5' to 3' order the following operatively linked elements: a reguladable promoter consisting of the Escherichia coli phage T5 promoter and two lac operator sequences, a ribosome binding site from the Escherichia coli phage T7g10, a translational start codon, a structural gene for IL-4 or an IL-4 mutein and downstream of that structural gene one transcription terminator. Furthermore the invention relates to Escherichia coli transformed with one or more vectors and use of the vector in a method of preparation of IL-4 and IL-4 muteins.