HIGH THROUGHPUT METHOD FOR FUNCTIONALLY CLASSIFYING PROTEINS IDENTIFIED USING A GENOMICS APPROACH

摘要

The present invention provides a method for functionally classifying a proteinthat is capable of unfolding due to a thermal change.The method comprises screening one or more of a multiplicity of differentmolecules for their ability to shift the thermal unfolding curveof the protein, wherein a shift in the thermal unfolding curve indicates thatthe molecule binds to the protein or affects the stability ina measurable way; generating an activity spectrum for the protein wherein theactivity spectrum reflects a set of molecules, from themultiplicity of molecules, that shift the thermal unfolding curve, of theprotein and therefore are ligands that bind to the protein, comparingthe activity spectrum for the protein to one or more functional referencespectrum lists; and classifying the protein according to the set ofmolecules in the multiplicity of different molecules that shift the thermalunfolding curve of the protein.