PRIMER AND PROBE FOR USE IN DETECTION OF MYCOBACTERIUM KANSASII AND METHOD FOR DETECTION OF MYCOBACTERIUM KANSASII USING THE SAME

摘要

An oligonucleotide which comprises a part or the entire of the nucleotide sequence depicted in SEQ ID NO:1, 2, 3 or 4 or a part or the entire of a sequence complementary to the nucleotide sequence and is capable of hybridizing with the nucleotide sequence for Mycobacterium kansasii gene; a primer and a probe for use in the detection of Mycobacterium kansasii comprising the oligonucleotide; and a method for detecting Mycobacterium kansasii using the primer and/or probe. The method for detecting Mycobacterium kansasii enables the detection of M. kansasii more rapidly and with higher accuracy compared with a conventional bacterium identification method performed by culture examination on a bacterium. Further, the method can exclude any false positive result for the diagnosis and can also detect and diagnose M. kansasii with higher accuracy compared with a diagnosis method performed by PCR using a conventional primer and/or probe. Still further, the method can quantify a M. kansasii cell.