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In vivo assay and molecular markers for testing the phenotypic stability.
In vivo assay and molecular markers for testing the phenotypic stability.
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机译:用于测试表型稳定性的体内测定法和分子标记物。
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摘要
An in vivo assay to measure anchorage-independent growth and phenotypic stability of a certain cell population comprising subcutaneous or intramuscular injection in a mammal of a cell suspension of articular chondrocytes in an iso-osmotic liquid, the same suspension comprising articular chondrocytes in an amount equivalent to at least 1 x 106 chondrocytes as applied to immune-deficient mice. The outcome is linked to molecular markers. The present invention further relates to DNA chips and diagnostic tools comprising the latter to predict the outcome of ACT. Antibodies raised against positive and negative markers of chondrocyte stability can also be used for quality control on the chondrocytes. Therapeutical composition comprising stable chondrocytes are very useful for tissue repair.
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机译:一种体内测定法,用于测量某些细胞群的不依赖于锚定的生长和表型稳定性,包括在哺乳动物体内皮下或肌肉内注射等渗液中的关节软骨细胞悬浮液,同一悬浮液包含等量的关节软骨细胞应用于免疫缺陷小鼠的至少1 x 106个软骨细胞。结果与分子标记有关。本发明进一步涉及DNA芯片和包括后者的诊断工具,以预测ACT的结果。针对软骨细胞稳定性的阳性和阴性标记物产生的抗体也可以用于软骨细胞的质量控制。包含稳定的软骨细胞的治疗组合物对于组织修复非常有用。
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