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METHOD FOR PRODUCING BEER CONTAMINATING LACTIC ACID BACTERIUM WHICH HAS BECOME DIFFICULT TO CULTURE AND TEST MEDIUM FOR BEER CONTAMINATING LACTIC ACID BACTERIUM
METHOD FOR PRODUCING BEER CONTAMINATING LACTIC ACID BACTERIUM WHICH HAS BECOME DIFFICULT TO CULTURE AND TEST MEDIUM FOR BEER CONTAMINATING LACTIC ACID BACTERIUM
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机译:啤酒污染乳酸菌培养及试验培养基的啤酒污染乳酸菌的生产方法
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摘要
It is intended to produce a beer contaminating lactic acid bacterial strain which has become difficult to culture under an artificial environment and to provide a biological material for devising an effective countermeasure against the bacterium. A method for producing a beer contaminating lactic acid bacterium which has become difficult to culture characterized by performing anaerobic culture by inoculating a lactic acid bacterium into a beer adjusted at a pH value of 4.0 to 5.5, and performing a heat treatment at 50 to 65ºC for 5 to 15 minutes after repeating an operation in which the obtained culture is inoculated into a beer adjusted at the pH value for 5 times or more; a method for producing a beer contaminating lactic acid bacterium which has become difficult to culture characterized by performing anaerobic culture by inoculating a lactic acid bacterium into a beer adjusted at a pH value of 4.0 to 5.5, and repeating an operation in which the obtained culture is inoculated into a beer adjusted at the pH value for more than 20 times; a test medium for a beer contaminating lactic acid bacterium comprising a beer and a powder MRS medium without agar added at a ratio of 16 g or less to 1L of the beer; and a method for testing a lactic acid bacterium in a beer characterized by using the test medium for a beer contaminating lactic acid bacterium are provided.
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