DEVELOPMENT OF MOLECULAR MARKER GENE RELATED WITH MEAT QUALITY USING STEAROYL- COA DESATURASE (SCD) GENE OF HANWOO

摘要

The present invention provides a marker gene involved in the development of beef cattle native country, yet only improve the meat quality of beef production resources relates to, intramuscular fat, which determines the beef and the quality of the meat (marbling score) and backfat thickness (backfat thickness), including the development of DNA markers for selection by the conductor and early diagnosis related to meat quality traits in beef and six kinds of improvement business and intended to take advantage of the high quality beef meat production. ; More specifically, the present invention is known as a key enzyme in the biosynthesis of unsaturated fatty acids of animals Stearoyl-CoA desaturase (SCD) in a particular single nucleotide polymorphism gene (single nucleotide polymorphism; SNP) in the Korean Native conductor and meat quality of the SCD gene intron In order to analyze the effects of transfection (Intron) 6 times and exon (Exon) 6 times, and intron (Intron) 7 times cloning a gene, and the gene structure including a region through sequence analysis Analysis of the results from two intron 6 each region, and it was found the two single nucleotide polymorphism (SNP) site in intron 7 area. And specific SNP markers found in these regions of the intron 7 is the fact that the significant association with the beef and meat quality traits of the conductor has been demonstrated in the present invention. ; In other words, beef SCD gene intron 7 single adenine (A) from the nucleotide sequence of a total size of 211bp containing the specific region guanine (G) found a single nucleotide polymorphism (SNP) site generated by base substitution. These single nucleotide polymorphism sequence site for PCR-SSCP (Single-Strand Conformation Polymorphism; single-chain form Conformation Polymorphism) method was detected using the three types of SNP genotypes (A / A, A / G and G / G). Among them, A / G genotype was found that there is a new and highly significant association of intramuscular fat, beef and backfat thickness. ; Thus, certain beef single SCD gene was developed by the present invention nucleotide polymorphism markers are very useful DNA markers for early selection of superior beef meat production is a good gogeupyuk regardless of age and gender beef as advanced molecular breeding technology compared to traditional selection methods that rely solely on conventional phenotypic selection by breeders Selection Tool can be used as an argument.