METHOD FOR PURIFICATING AN ACETYLCHOLINESTERASE(ACHE) FROM HONEYBEE(APIS MELLIFERA) HEADS USING ANTIBODY AFFINITY CHROMATOGRAPHY

摘要

invention antibody affinity chromatography with bee the tofu-derived acetylcholinesterase claim atmospheres (AChE) of relates to a purification method, and more particularly through the extraction of the antigen with AChE OSU 6B affinity chromatography with epoxy activated Sepharose by using the affinity of the antibody obtained from the rabbit (Epoxy-activated Sepharose 6B Affinity Chromatography) from bee head relates to the purification of enzyme activity and bee than affinity chromatography using an antibody to the protein content is supplied to the high AChE acetylcholinesterase tofu-derived claim atmospheres (AChE). ; The present invention with the prior in line with the purification steps were used to obtain a high purity AChE, yet effective, simple and economical at the same time it can be mass than conventional provide a stable high activity of the enzyme required for the kit used in the method of detecting insect pesticide residue derived AChE has the effect of increasing the feed rate of.