THE DEVELOPMENT OF HUMAN BONE MARROW STROMAL CELLS TO PROMOTE HUMAN EMBRYONIC STEM CELLS PROLIFERATION AND DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO EMBRYOID BODIES

摘要

A kind of method, it is arranged to produce human embryonic stem cell for cultivating human embryo stem cell, it is more safely applied to the human body compared with using mice embryonic fibrocyte as a feeder cells by using Human bone marrow stromal's unit, to be effectively applied to human cell's therapy by the embryoid body that human embryonic stem cell generates. The method comprising the steps of: :(a) it is inoculated on the feeder cells including Human bone marrow stromal's unit to human embryonic stem cell; (b) after increasing the culture solution to the human embryonic stem cell given for human embryonic stem cell, it is cultivated; (c) it is washing after the product that there is the step of free serum culture solution for human embryonic stem cell (b) to obtain, add culture solution again for human embryonic stem cell thereto and then is cultivating it. In method, Human bone marrow stromal's unit prepares by delivering radiation to the Human bone marrow stromal's unit being collected and cultivating them and has the sustentacular cell layer bottom of on.