DISCRIMINATION METHOD OF JIN-DO NATIVE PLANTS PUNGRAN

摘要

The present invention relates to a method of differentiating mountain native plants Neofinetia falcata progress. ; differential method of magnitude acid Neofinetia falcata native plants of the present invention and magnitude acid Neofinetia falcata DNA extraction step of extracting DNA from the magnitude Neofinetia falcata acid; Amplifying the DNA of the extracted magnitude acid Neofinetia falcata by RAPD-PCR analysis using primers of SEQ ID NO: 1 or SEQ ID NO: 2 Neofinetia falcata magnitude acid and DNA amplification step; Electrophoresis, and the genomic DNA of the amplified magnitude acid Neofinetia falcata next to the band magnitude acid Neofinetia falcata confirmation confirming the position by using the ultraviolet band reader; After preparing a differential target Neofinetia falcata to distinguish, and differentiate the target Neofinetia falcata DNA extraction step of extracting the DNA of the target Neofinetia falcata discrimination; Amplifying the DNA of the extracted target differential Neofinetia falcata by RAPD-PCR analysis using primers of SEQ ID NO: 1 or SEQ ID NO: 2 Differential Neofinetia falcata target DNA and amplification steps; Electrical the genomic DNA of the amplified target Neofinetia falcata differential electrophoretic band and then differential target Neofinetia falcata confirmation confirming the position by using the ultraviolet band reader; By comparing the magnitude of the differential target band position and band position Neofinetia falcata of San Neofinetia falcata differentiation stage of differentiation of progress acid Neofinetia falcata; consists of ; According to the present invention, it is difficult to distinguish with the naked eye magnitude San Neofinetia falcata there is provided a method that can easily be differentiated, there is provided a method of differentiating reproducible acid Neofinetia falcata progress.