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Transgenic plants using superoxide dismutase gene of Lilium hybrid cultivar Marco Polo

机译:利用百合杂种马可波罗的超氧化物歧化酶基因转基因植物

摘要

The present invention is Lico Marcopolo Activity in transgenic potatoes prepared using plant transfer vectors recombined so that cDNA is expressed in the forward and reverse directions using Cu / Zn lily-superoxide dismutase (LSOD) genes expressed in scleroderma. The distribution of oxygen species is different, and their effects show opposite growth and tuber formation rates, and have a great effect on the vitrification rate during in-flight culture.;The Cu / Zn LSOD gene of the present invention increases the H 2 O 2 content and decreases the superoxide radical content in the plant when overexpressed, which inhibits growth in potatoes, resulting in a very good initial tuberization rate while shortening the length between the shoot and the node. By introducing a gene encoding the same into a plant cell, the development of a crude plant that can shorten the growth period and the reverse expression thereof can provide a potato plant of all species that can increase the growth period. In addition, the vitrification rate, which is a problem during mass cultivation in the cabin, can be drastically reduced, thereby increasing the purifying rate of aseptic seedlings and lowering the mortality rate during sowing of the in-flight plants, and can provide a transformed potato plant that is resistant to excessive moisture stress environment. .;Superoxide Dismutase, LSOD, Potato, Transgenic Potato Plant
机译:本发明是使用重组的植物转移载体制备的转基因马铃薯中的Lico Marcopolo活性,从而利用硬皮病中表达的Cu / Zn百合超氧化物歧化酶(LSOD)基因以正向和反向表达cDNA。氧种类的分布不同,其作用显示相反的生长和块茎形成速率,并且对飞行中培养期间的玻璃化速率有很大的影响。;本发明的Cu / Zn LSOD基因增加了H 2 O 2 含量并过表达会降低植物中的超氧自由基含量,这会抑制马铃薯的生长,从而导致很好的初始块茎率,同时缩短了芽和茎之间的长度。节点。通过将编码该基因的基因导入植物细胞,可以缩短生长期并进行逆向表达的粗制植物的发育可以提供可以延长生长期的所有种类的马铃薯。另外,可以显着降低在机舱中进行大规模培养时出现的玻璃化率,从而提高无菌幼苗的净化率并降低飞行中植物播种时的死亡率,并且可以提供耐过度水分胁迫环境的马铃薯植株。 。;超氧化物歧化酶,LSOD,马铃薯,转基因马铃薯植株

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