58 Monoclonal Antibody 5E8 specific to West Nile Virus its Hybridoma and Enzyme-linked Immunosorbent Assay

摘要

The present invention relates to a diagnostic method using the fused cell lines and the monoclonal antibodies that secrete monoclonal antibodies binding to the region of the outer covering E367 alanine E protein of West Nile virus. ; The object of the invention is coupled to E367 alanine portion of the shell E protein of West Nile virus, providing a fused cell line secreting a neutralizing monoclonal antibody 5E8 which inhibits the virus in infection sensitive cells, and is secreted from here that the 5E8 monoclonal antibody utilized to provide a new diagnostic method for rapidly detecting the West Nile virus neutralizing antibodies. ; More specifically, the fused cell lines of the present invention is prepared by the cell fusion between the West Nile B immune cells of said immune to viral antigens valves (Balb / c) mice with SP2 / 0 myeloma-derived cell, West Nile virus E sheath characterized in that the secreted monoclonal antibodies that bind to a neutralization site of protein E367 alanine. Also it provides a diagnostic method which can be rapidly detected in the binding competitively enzyme immunoassay neutralizing antibodies to West Nile virus infection time by using the monoclonal antibody 5E8. ; Monoclonal antibody produced by the fused cell line of the present invention can be used to hold it and the ability to neutralize the West Nile Virus, as well as a diagnostic antibody therapeutic antibody development. Diagnostic method of the novel present invention can be used for serum diagnosis, regardless of the animal species, West Nile can quickly measure the infection defense antibody levels in the vaccinated individual in order to prevent virus. ; West Nile virus, monoclonal antibodies, neutralizing antibodies, competitively enzyme-linked immunoassay