Expression vector for transgenesis of silkworms and transgenic silkworm sustained the fifth instar larval period

摘要

PURPOSE: An expression vector for transgenesis of silkworms and transgenic silkworm sustained for fifth instar larval period, containing the same are provided, thereby increasing the weight and size of larvae, pupae and silkworms due to the instar larval period sustainment. CONSTITUTION: The expression vector pAcIE1-EGT for transgenesis of silkworms is produced by the steps of: (1) cloning IE1 gene of AcNPV into a vector pBluescripSK(-); (2) removing 1.3 kb HindII fragment to prepare pAcIE1; (3) inserting SV40 poly(A) site derived from animals into the vector pAcIE1; and (4) inserting EGT gene derived from BmNPV-K1 into the vector pAcIE1 under regulation of IE1 gene promoter. The transgenic silkworm sustained for fifth instar larval period is produced by inserting the recombinant expression vector pAcIE1-EGT into silkworms.