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METHOD FOR CULTIVATION OF LAWSONIA INTRACELLULARIS BACTERIA IN MCCOY CELLS AND APPLICATION OF MCCOY CELLS FOR EXTRACELLULAR CULTIVATION OF LAWSONIA INTRACELLULARIS BACTERIA
METHOD FOR CULTIVATION OF LAWSONIA INTRACELLULARIS BACTERIA IN MCCOY CELLS AND APPLICATION OF MCCOY CELLS FOR EXTRACELLULAR CULTIVATION OF LAWSONIA INTRACELLULARIS BACTERIA
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机译:在MCCOY细胞中培养松果体细菌的方法和MCCOY细胞在细胞外培养松果体细菌中的应用
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摘要
Cultivating Lawsonia intracellularis (LI) bacteria comprises: (a) incubating the bacteria at an oxygen concn. of 18% and (b) maintaining the bacteria in suspension, to cultivate the bacteria. Also claimed are: (1) a method for cultivating LI bacteria comprising: (a') inoculating culture cells with an inoculum comprising LI bacteria so as to infect the cells with the bacteria and (b') incubating the infected cells at a temp. of 36 -38 [deg]C in an oxygen concn. of 0-8% while agitating the infected cells so as to cultivate the LI while maintaining the infected cells in suspension; (2) a method for producing an attenuated LI strain comprising: (i) obtaining culture cells infected with LI bacteria, (ii) incubating the infected cells at an oxygen concn. of 0-18%, (iii) agitating the infected cells so as to cultivate the bacteria while maintaining the infected cells in suspension, (iv) passaging at least a portion of the cultivated bacteria and (v) selecting for an attenuated strain to provide an attenuated LI bacteria; (3) a vaccine for inducing an immune response to LI bacteria in an animal comprising an attenuated strain of LI in a carrier, where the attenuated strain is in amt. effective to produce an immune response.
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