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首页> 外国专利> SLIM Mapping quantitative trait loci of chromosome 17 and its application to marker assisted selection

SLIM Mapping quantitative trait loci of chromosome 17 and its application to marker assisted selection

机译：17号染色​​体的SLIM定位定量性状基因座及其在标记辅助选择中的应用

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摘要

1.method for selecting the first pigs using the marker locus for quantitative indication associated with signs of growth, a u043du0430u043bu0438u0447u0438u00a0 is per definition howling pig locus for located in chromosome 17, from about 70 to about 104 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection the first move u0442u043du043eu0433u043e containing the locus and the locus for the quantitative choice ofassociated with the signs of growth.;2. method for 1, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c restriction site that is selected from the group consisting of Dde i, Msp i, Nae i, Afl iii, Alw ni, Bse ri, Taa i, Mse i, Bst ui, Bcc i, Taq i and Mnl i.;3.method for selecting the first pigs using the marker locus for quantitative indication associated with the u0433u043bu0438u043au043eu043bu0438u0442u0438u0447u0435u0441u043au0438u043c capacity and the average content of lactate, etc. u0435u0434u0443u0441u043cu0430u0442u0440u0438u0432u0430u044eu0449u0438u0439 definition u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located in chromosome 17, from about 80 to about 84 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044b m, marker selection of the first animal containing the locus.and the choice of locus for quantitative indication associated with the u0433u043bu0438u043au043eu043bu0438u0442u0438u0447u0435u0441u043au0438u043c capacity and the average content of lactate.;4. method for article 3, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Nae i restriction site.;5. method for article 3, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Afl iii restriction site.;6.method for selecting the first pigs using the marker locus for quantitative indication associated with color, fat meat "(from hunter" medium u043fu043eu0442u0435u0440u00a0u043cu0438 due to spill u0430u043du0438u00a0 juice and / or fat meat "(from minolta" to the definition of u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located in chromosome 17, from approximately 83 to 88 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c markerthe selection of the first animal containing the locus, and the choice of locus for quantitative indication associated with color, fat meat "(from hunter" average u0442u0435u0440u00a0u043cu0438 due to u0432u044bu0442u0435u043au0430u043du0438u00a0 juice and / or fat meat "(from minolta".;7. method for p.6, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Afl iii restriction site.;8. method for p.6, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Alw ni.;9. method for p.6, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Bse ri.;10.method for selecting the first pigs using the marker locus for quantitative indication associated with the length and / or u0445u0440u0435u0431u0442u043eu0432u044bu043c u0448u043fu0438u043au043eu043c u043fu043eu00a0u0441u043du0438u0447u043du043eu0433u043e division, u043fu0440u0435u0434u0443u0441u043cu0430u0442u0440u0438u0432 u0430u044eu0449u0438u0439 definition u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located at chromosome 17 in the area from about 83 to about 85 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker the selection of the first animal containing the locus.and the choice of locus for quantitative indication associated with the length and / or u0445u0440u0435u0431u0442u043eu0432u044bu043c u0448u043fu0438u043au043eu043c u043fu043eu00a0u0441u043du0438u0447u043du043eu0433u043e division.;11. way on p.10, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Afl iii restriction site.;12. way on p.10, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Alw ni.;13.method for selecting the first pigs using the marker locus for quantitative indication associated with the u0445u0440u0435u0431u0442u043eu0432u044bu043c u0448u043fu0438u043au043eu043c u043fu043eu00a0u0441u043du0438u0447u043du043eu0433u043e division for the in the first u043du0430u043bu0438u0447u0438u00a0 locus for pigs, located at chromosome 17 in the area from about 85 to 90 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection order u0430u043du043du043eu0433u043e first animal containing the locus.and the choice of locus for quantitative indication associated with the u0445u0440u0435u0431u0442u043eu0432u044bu043c u0448u043fu0438u043au043eu043c u043fu043eu00a0u0441u043du0438u0447u043du043eu0433u043e division.;14. way on p.13, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Alw ni.;15. way on p.13, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Bse ri.;16. way on p.13, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Taa i restriction site.;17.method for selecting the first pigs using the marker locus for quantitative indication associated with signs of growth and fat content by definition at u043bu0438u0447u0438u00a0 at the first locus for pigs, located in chromosome 17, from approximately 88 to about 91 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection of pe u0440u0432u043eu0433u043e animal containing the locus.and the choice of locus for quantitative indication associated with signs of growth and fat content.;18. way to p.17, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Mnl i restriction site.;19. way to p.17, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Taa i restriction site.;20.method for selecting the first pigs using the marker locus for quantitative indication associated with the u043fu043eu0442u0435u0440u00a0u043cu0438 in thermal processing, which u043eu043fu0440u0435u0434u0435u043bu0435u043du0438 (e u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located at chromosome 17 in the area from about 97 to 100 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection u0443u043au0430u0437u0430u043du043du043eu0433 the first animal containing the locus.and the choice of locus for quantitative indication associated with the u043fu043eu0442u0435u0440u00a0u043cu0438 during thermal processing.;21. way on p.20, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Mse i restriction site.;22. way on p.20, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Bst ui.;23. way on p.20, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Bcc i restriction site.;24. way on p.20, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Taq i restriction site.;25.method for selecting the first pigs using the marker locus for quantitative indication associated with color, fat meat "(from hunter" fat meat "(from minolta" medium u043fu043eu0442u0435u0440u00a0 mi due to u0432u044bu0442u0435u043au0430u043du0438u00a0 juice and / or u043cu00a0u0433u043au043eu0441u0442u044cu044e, involving the definition of u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs.located in chromosome 17, from about 100 to about 104 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker, the selection of the first animal containing this locus, and the choice of locus for quantitative indication associated with color, fat meat "(from hunter" fat meat "(from minolta" medium u043fu043eu0442u0435u0440u00a0u043cu0438 due to u0432u044bu0442u0435u043au0430u043du0438u00a0 juice and / or u043cu00a0u0433u043au043eu0441u0442u044cu044e.;26. way on p.25, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Taq i restriction site.;27. way on p.25, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 server i restriction site.;28. way on p.25, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Alw ni.;29.method for selecting the first pigs using the marker locus for quantitative indication associated with signs of growth, a u043du0430u043bu0438u0447u0438u00a0 is per definition howling pig locus for located in chromosome 17, from about 103 to about 107 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection the first move u0442u043du043eu0433u043e containing the locus and the locus for the quantitative choice ofassociated with the signs of growth.;30. the way in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 p.29, Nae i restriction site.;31. method for p.29, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Alw ni.;32. method of identification of u0430u043bu043bu0435u043bu00a0, associated with signs of growth, which provides u0432u0437u00a0u0442u0438u0435 animal has samples of tissue or body fluid u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u044e the dna present in the sample containing the chromosomes 17 from about 70 to about 107 cm, and the detection of the specified section of the polymorphous marker chromosome and, u0444u0435u043du043eu0442u0438u043fu0438u0447u0435u0441u043au043eu0439 variability associated with signs of growth.;33.method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 genetic marker, fit u0434u043bu00a0 identification and selection of animals on the basis of their signs of growth, which is the u0432u0437u00a0u0442u0438u0435 well u0438u0432u043eu0442u043du044bu0445 samples of tissue or body fluid containing dna, u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u044e dna present in the sample on the chromosome 17 from about 70 to about 107 with m in the sample from the first animalthe definition of u043du0430u043bu0438u0447u0438u00a0 polymorphous u0430u043bu043bu0435u043bu00a0 in the sample through the sample u0441u0440u0430u0432u043du0435u043du0438u00a0 reference model or the sequence u043au043eu0440u0440u0435u043bu00a0u0446u0438u044e swings of growth, fat content or quality u043cu00a0u0441u0430 from the animals with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c u0430u043bu043bu0435u043bu0435u043c, u0431u043bu0430u0433u043eu0434u0430u0440u00a0 which the allel can be used as genetic markers the sign of the marker in this group, u043fu043eu043fu0443u043bu00a0u0446u0438u0438 or form.;34. method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 genetic marker, fit u0434u043bu00a0 identification and selection of animals on the basis of the quality of u043cu00a0u0441u0430 or growth for the op u0440u0435u0434u0435u043bu0435u043du0438u0435 polymorphous u0430u043bu043bu0435u043bu00a0 in acceptable imbalance u0441u0446u0435u043fu043bu0435u043du0438u00a0 with marker on p.33.;35.method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 genetic marker, fit u0434u043bu00a0 identification and selection of animals on the basis of the quality of u043cu00a0u0441u0430, fat content or growth, u043fu0440u0435u0434u0443u0441u043cu0430u0442u0440 u0438u0432u0430u044eu0449u0438u0439 u0432u0437u00a0u0442u0438u0435 from the animal tissue or body fluid samples containing dna, u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u044e dna, located on the chromosome 17 from about 70 to about colo 90 or from about 97 to about 107.5 cm in the first animal model, the definition of u043du0430u043bu0438u0447u0438u00a0 polymorphous u0430u043bu043bu0435u043bu00a0 in the sample through the sample, the reference sample or u0441u0440u0430u0432u043du0435u043du0438u00a0 the sequence u043au043eu0440u0440u0435u043bu00a0u0446u0438u044e variability in growth, fat content or quality u043cu00a0u0441u0430 from the animals with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c u0430u043bu043bu0435u043bu0435u043c,what the u0431u043bu0430u0433u043eu0434u0430u0440u00a0 allel can be used as a genetic marker of the symptoms in this group, u043fu043eu043fu0443u043bu00a0u0446u0438u0438 or form.;36. method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 genetic marker, fit u0434u043bu00a0 identification and selection of animals on the basis of the quality of u043cu00a0u0441u0430 or growth for the op u0440u0435u0434u0435u043bu0435u043du0438u0435 polymorphous u0430u043bu043bu0435u043bu00a0 in acceptable imbalance u0441u0446u0435u043fu043bu0435u043du0438u00a0 with marker on p.35.;37. the way in which the phase u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 on p.35, choose from the group including u0440u0435u0441u0442u0440u0438u043au0446u0438u043eu043du043du043eu0433u043e fragment length polymorphism analysis (RFLP), u043cu0438u043du0438u0441u0435u043au0432u0435u043du0438u0440u043eu0432u0430u043du0438u0435, MALD - TOF, my mr. u0435u0442u0435u0440u043eu0434u0443u043fu043bu0435u043au0441u043du044bu0439 analysis, analysis of polymorphism u043au043eu043du0444u043eu0440u043cu0430u0446u0438u0438 one chain (SSCP), gel electrophoresis in u0434u0435u043du0430u0442u0443u0440u0438u0440u0443u044eu0449u0435u043c gradient (DGGE) and gel electrophoresis in temperature gra u0434u0438u0435u043du0442u0435 (TGGE).;38. way on p.35, in which the animal u00a0u0432u043bu00a0u0435u0442u0441u00a0 pig.;39. method for u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u00a0 p.35, which includes the choice of upper and lower primer capable of u0430u043cu043fu043bu0438u0444u0438u0446u0438u0440u043eu0432u0430u0442u044c the site of chromosome 17.;40.method for selecting the first pigs using the marker locus for quantitative indication associated with an average daily weight gains, by definition we u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located in chromosome 17, from about 70 to about 72 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection u0443u043au0430u0437u0430u043du043d wow the first animal containing the locus.and the choice of locus for quantitative indication associated with an average daily weight gains.;41. the way in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 p.40, Dde i restriction site.;42. the way in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 p.40, Msp i restriction site.;43.method for selecting the first pigs using the marker locus for quantitative indication associated with a scoring assessment of marbling u043cu00a0u0441u0430 by definition we u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located at chromosome 17 in the area from about 72 to 80 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection u0443u043au0430u0437u0430u043du043d wow the first animal containing the locus.and the choice of locus for quantitative indication associated with the point of u043cu00a0u0441u0430 marbling.;44. way on p.43, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Msp i restriction site.;45. way on p.43, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Nae i restriction site.;46.method for selecting the first pigs using the marker locus for quantitative indication associated with the average content of u0445u0440u0435u0431u0442u043eu0432u043eu0433u043e disguise with oprah of u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located at chromosome 17 in the area from about 85 to 90 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection guidance drop the first animal containing the locus.and the choice of locus for quantitative indication associated with the average content of u0445u0440u0435u0431u0442u043eu0432u043eu0433u043e disguise.;47. method for p.46, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Alw ni.;48. method for p.46, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Bse ri.;49. the way in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 p.46, Taa i restriction site.;50.method for selecting the first pigs using the marker locus for quantitative indication associated with the average content of u0445u0440u0435u0431u0442u043eu0432u043eu0433u043e salt pork, ham back there "hunter" and / or an eye rock "minolta" to the definition of u043du0430u043bu0438u0447u0438u00a0 at the first locus for pigs, located at chromosome 17 in the area from about 97 to 99 cm and about genetically u0441u0432u00a0u0437u0430u043du043du043eu0433 on the u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker, the selection of the first animalcontaining the locus, and the choice of locus for quantitative indication associated with the average content of u0445u0440u0435u0431u0442u043eu0432u043eu0433u043e salt pork, ham back there "hunter" and / or "minolta ham back there.;51. way to p.50, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 Mse i restriction site.;52. way to p.50, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Bst ui.;53.method for selecting the first pigs using the marker locus for quantitative indication associated with the thickness u0445u0440u0435u0431u0442u043eu0432u043eu0433u043e disguise "Aloca" position p2, u043fu0440u0435u0434u0443u0441u043cu0430u0442u0440u0438u0432u0430u044eu0449u0438 "the first pig locus for located in chromosome 17, from about 100 to about 103 cm and genetically u0441u0432u00a0u0437u0430u043du043du043eu0433u043e with u043fu043eu043bu0438u043cu043eu0440u0444u043du044bu043c marker selection order u0430u043du043du043eu0433u043e first animal containing the locus.and the choice of locus for quantitative indication associated with the thickness u0445u0440u0435u0431u0442u043eu0432u043eu0433u043e disguise "Aloca" in the position p2.;54. method for p.53, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 restriction site Alw ni.;55. the way in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 p.53, Taq i restriction site.;56. method for p.53, in which the marker u00a0u0432u043bu00a0u0435u0442u0441u00a0 server i restriction site.
机译：1，使用标记基因座来选择与生长迹象相关的定量指示来选择第一批猪的方法，根据定义，第17号染色​​体上的 u043d u0430 u043b u0438 u0447 u0438 u00a0是how叫猪基因座70至约104厘米，并在基因上 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u043e u0433 u043e与 u043f u043e u043b u0438 u043c u043e u04e u0440 u0444 u043d u044b u043c标记的选择第一步，包含基因座和用于定量选择的与生长迹象相关的基因座; 2。 1的方​​法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 u043f u043e u043b u0438 u043c u043e u0440 u0444 u0434 u043d u044b u043c限制位点选自Dde i，Msp i，Nae i，Afl iii，Alw ni，Bse ri，Taa i，Mse i，Bst ui，Bcc i，Taq i和Mnl i.3。选择第一个方法使用标记位点进行定量指示的猪与 u0433 u043b u0438 u043a u043e u043b u0438 u0442 u0438 u0447 u0435 u0441 u043a u0438 u043c的容量和平均乳酸含量等相关。 u0435 u0434 u0443 u0441 u043c u0430 u0442 u0440 u0438 u0432 u0430 u044e u0449 u0438 u0439定义 u043d u0430 u043b u0438 u0447 u0438 u00a0在第一个位置对于位于第17号染色​​体上的猪（约80到84厘米），并通过遗传方式 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e和 u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u044b m，第一个包含基因座和菜的动物的标记选择定量指示的场所与 u0433 u043b u0438 u043a u043e u043b u0438 u0442 u0438 u0447 u0435 u0431 u0431 u043a u0438 u043c的容量和平均乳酸含量相关; 4。第3条的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Nae i限制位点。5。第3条的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Afl iii限制位点; 6。使用标记位点选择第一批猪的方法，用于与颜色相关的定量指示，脂肪肉（来自猎人）介质 u043f u043e u0442 u0435 u0440 u00a0 u043c u0438由于溢出 u0430 u043d u0438 u00a0果汁和/或脂肪肉（来自美能达”定义为位于第17个染色体的第一个猪位处 u043d u0430 u043b u0438 u0447 u0438 u00a0的定义，从大约83到88 cm处，并且在遗传上 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e和 u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u044b u043c标记选择包含该基因座的第一只动物，并选择用于定量指示的基因座与颜色相关的脂肪肉（来自“猎人”平均 u0442 u0435 u0440 u00a0 u043c u0438由于 u0432 u044b u0442 u0435 u043a u0430 u043d u0438 u00a0果汁和 /或脂肪肉“（来自minolt a “。;7。第6页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Afl iii限制性位点。第6页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Alw ni。; 9。 p.6的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Bse ri ;; 10.方法，使用标记位点选择与之相关的定量标记的第一头猪长度和//或 u0445 u0440 u0435 u0431 u0442 u043e u0432 u044b u043c u0448 u043f u0438 u043a u043e u043c u043f u043e u00a0 u0441 u043d u0438 u0447 u043d u043e u0433 u043e部门， u043f u0440 u0435 u0434 u0443 u0441 u043c u0430 u0442 u0440 u0438 u0432 u0430 u044e u0449 u0438 u0439定义 u043d u0430 u0438 u0447 u0438 u00a0位于猪的第一个基因座，位于约83至约85 cm区域的第17号染色​​体上，并且在遗传上 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 使用带有 u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u044b u043c标记的u043e选择包含该基因座的第一只动物，并选择与长度和 /相关的定量指示基因座或 u0445 u0440 u0435 u0431 u0442 u043e u043 2 u044b u043c u0448 u043f u0438 u043a u043e u043c u043f u043e u00a0 u0441 u043d u0438 u0447 u043d u043e u0433 u043e部门。; 11。第10页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Afl iii限制性位点。第10页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Alw ni。; 13。使用标记位点选择第一头猪的方法，用于与 u0445 u0440 u0435 u0431 u0442 u043e u0432 u044b u043c u0448 u043f u0438 u043a u043e u043c u043f u043e u00a0 u0441 u043d u0438 u0447 u043d u043d u043e划分为第一个 u043d u0430 u043b u0438 u0447 u0438 u00a0猪的场所，位于大约85至90厘米区域的17号染色​​体上，并通过 u043f u043e u043b u0438 u043c u043e u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e u0440 u0444 u043d u044b u043c标记选择顺序 u0430 u043d u043d u043e u0433 u043e包含该基因座的第一只动物，以及与 u0445 u0440 u0435 u0431 相关的定量指示基因的选择u0442 u043e u0432 u044b u043c u0448 u043f u0438 u043a u043e u043e u043c u043f u043e u00a0 u0441 u043d u0438 u0447 u043d u043d u043e u0433 u043e分区。; 14。第13页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Alw ni。; 15。第13页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Bse ri。; 16。第13页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Taa i限制位点; 17。使用标记位点选择与迹象相关的定量指示的第一头猪的方法定义为位于第17个染色体的第一个猪位处 u043b u0438 u0447 u0438 u00a0的生长和脂肪含量，从大约88厘米到大约91厘米，遗传上为 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e与 u043f u043e u043b u0438 u043c u043e u043e u0440 u0444 u043d u043b u043b u043c标记的pe u0440 u0432 u043e u043e u0433 u043e动物的标记选择以及与生长和脂肪含量迹象相关的定量指标的选择; 18。通往第17页的方式，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Mnl i限制性位点。转到第17页，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Taa i限制位点; 20。使用标记位点选择第一头猪的方法，用于与 u043f u043e u0442 u0435 u0440 u00a0 u043c u0438在热处理中，其中 u043e u043f u0440 u0435 u0434 u0435 u043b u0435 u043d u0438（e u043d u0430 u043b u0438 u0447 u0438 u00a0位于第一个猪位，位于约97至100 cm区域的第17号染色​​体上，并且在遗传上 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u044b u043b u043c标记选择 u0443 u043a u0430 u0437 u0430 u043d u043d u043d u043e u043e u0433与热处理过程中与 u043f u043e u0442 u0435 u0440 u00a0 u043c u0438相关的定量指示的位置的选择; 21.在第20页上，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Mse istrictio n网站。; 22。第20页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Bst ui。; 23。第20页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Bcc i限制位点。24。第20页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Taq i限制位点; 25。使用标记位点选择第一批猪的方法，用于与颜色相关的定量指示，由于 u0432 u044b u0442 u0435 u043a u0430 u043d ，脂肪肉“（来自猎人”脂肪肉“（来自美能达”介质 u043f u043e u0442 u0435 u0440 u00a0 mi u0438 u00a0果汁和 /或 u043c u00a0 u0433 u043a u043e u0431 u0441 u0442 u044c u044e，其中涉及 u043d u0430 u043b u0438 u0447 u0447 u0438 u00a0的定义猪位于第17号染色​​体上，约100到约104厘米，并在遗传上 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e和 u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u044b u043c标记，选择包含该基因座的第一只动物，以及选择与颜色相关的定量指示基因的场所，脂肪肉（来自猎人）“脂肪肉”（来自美能达， ”中 u043f u043e u0442 u0435 u0440 u00a0 u043c u0438，因为 u0432 u044b u0442 u0435 u043a u0430 u043d u0438 u00a0果汁和 /或 u043c u00a0 u0433 u043a u043e u0441 u0442 u0442 u044c u044e。; 26。第25页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Taq i限制位点。; 27。第25页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0服务器i限制站点。第25页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Alw ni。; 29。方法，使用标记位点选择与迹象相关的定量指示的第一头猪根据生长的定义， u043d u0430 u043b u0438 u0447 u0438 u00a0位于第17号染色​​体，从大约103厘米到大约107厘米，从遗传上讲是pig叫猪基因座，并且在基因上 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e，其中 u043f u043e u043b u0438 u043c u043e u043e u0440 u0444 u043d u044b u043c标记选择包含位置的第一个动作 u0442 u043d u043e u0433 u043e以及与生长迹象相关的定量选择的场所。; 30。标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0第29页的方式，Nae i限制位点; 31。第29页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Alw ni。; 32。识别 u0430 u043b u043b u0435 u043b u00a0的方法，与生长迹象相关，可提供 u0432 u0437 u00a0 u0442 u0438 u0435动物具有组织或体液样本 u0430 u043c u043f u043b u0438 u0444 u0438 u043a u0430 u0446 u0438 u044e样本中存在的dna包含约70至约107 cm的第17号染色​​体，并检测多态性标记染色体和， u0444 u0435 u043d u043e u0442 u0438 u043f u0438 u0447 u0435 u0441 u043a u043e u0439与生长迹象相关的变异性; 33。方法 u043e u043e u043f u0440 u0435 u0434 u0435 u043b u0435 u043d u0438 u00a0遗传标记，适合 u0434 u043b u00a0根据其生长迹象对动物进行识别和选择，即 u0432 u0437 u00a0 u0442 u0438 u0435井 u0438 u0432 u043e u0442 u043d u044b u0445包含dna， u0430 u043c u043f u043b u0438 u0444 u0438 u043a u043a u0430 u0446 u0438 u044e的dna组织或体液样本当下 在第一个动物的样本中，第17号染色​​体样本中的m从70到约107，其中 u043d u0430 u043b u0438 u0447 u0438 u00a0多态 u0430 u043b u043b u0435 u043b 样本中的u00a0通过样本 u0441 u0440 u0430 u0432 u043d u0435 u043d u0438 u00a0参考模型或序列 u043a u043e u0440 u0440 u0435 u043b u043b u00a0 u0446 u0438 u044e来自具有 u043f u043e u043b u0438 u043c u043e u0440 u0444 u0444 u043d u044b u043c u043c u04c u0430 u043b u043b u043b u0435的动物的生长，脂肪含量或质量的波动 u043b u0435 u043c， u0431 u043b u0430 u0433 u043e u0434 u0430 u0440 u00a0可以将等位基因用作该组中标记的标志，即 u043f u043e u043f u0443 u043b u00a0 u0446 u0438 u0438或表格。; 34。方法 u043e u043f u0440 u0435 u0434 u0435 u043b u0435 u043d u0438 u00a0遗传标记，适合 u0434 u043b u00a0并根据 u043c u00a0 u0441 u0430或op u0440 u0435 u0434 u0435 u043b u0435 u043d u043d u0438 u0435多态 u0430 u043b u043b u0435 u043b u00a0处于可接受的失衡状态 u0441 u0446 u0435 u043f u043b u0435 u043d u0438 u00a0在第33页带有标记;; 35。方法 u043e u043f u0440 u0435 u0434 u0435 u043b u0435 u043d u0438 u00a0遗传标记，适合 u0434 u043b u00a0根据 u043c u00a0 u0441 u0430的质量，脂肪含量或生长， u043f u0440 u0435 u0434 u0444 u0443 u0441 u043c u0430 u0430 u0442 u0440 u0438 u0432 u0430 u044e u0449 u0438 u0439 u0432 u0437 u00a0 u0442 u0438 u0435来自包含dna， u0430 u043c u043f u043b u0438 u0444 u0438的动物组织或体液样本 u043a u0430 u0446 u0438 u044e dna，位于第70号染色体上，从大约70到abo ut colo 90或在第一个动物模型中从约97到约107.5厘米，样本中 u043d u0430 u043b u0438 u0447 u0438 u00a0多形 u0430 u043b u043b u0435 u043b u00a0的定义通过样本，参考样本或 u0441 u0440 u0430 u0432 u043d u0435 u043d u0438 u00a0序列 u043a u043e u0440 u0440 u0435 u043b u00a0 u0446 u0438 u044e的可变性来自具有 u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u043d u043b u043c u0430 u043b u043b u043b u0435 u043b的动物的生长，脂肪含量或质量 u043c u00a0 u0441 u0430 u0435 u043c， u0431 u043b u0430 u0433 u043e u0434 u0430 u0440 u00a0 allel可以用作该组症状的遗传标记， u043f u043e u043f u043f u0443 u043b u00a0 u0446 u0438 u0438或表格。; 36。方法 u043e u043f u0440 u0435 u0434 u0435 u043b u0435 u043d u0438 u00a0遗传标记，适合 u0434 u043b u00a0并根据 u043c u00a0 u0441 u0430或op u0440 u0435 u0434 u0435 u043b u0435 u043d u043d u0438 u0435多态 u0430 u043b u043b u0435 u043b u00a0处于可接受的失衡状态 u0441 u0446 u0435 u043f u043b u0435 u043d u0438 u00a0，在第35页有标记;; 37。在第35页上的阶段 u043e u043f u0440 u0435 u0434 u0435 u043b u0435 u043d u0438d u0438 u00a0的方式中，从包括以下内容的组中选择 u043a u0446 u0438 u043e u043d u043d u043e u0433 u043e片段长度多态性分析（RFLP）， u043c u0438 u043d u0438 u0441 u0435 u043a u0432 u0432 u0435 u043d u0438 u0440 u043e u0432 u0430 u043d u0438 u0435，MALD-TOF，我的先生 u0435 u0442 u0435 u0440 u043e u0434 u0443 u043f u043b u0435 u043a u0441 u043d u043b u043b u0439分析，多态性分析 u043a u043e u043d u043d u0444 u043e u0440 u043c u0430 u0446 u0438 u0438一条链（SSCP），在 u0434 u0435 u043d u0430 u0442 u0443 u0440 u0438 u0440 u0443 u044e u0449 u0435 u043c梯度（DGGE）中进行凝胶电泳，并在温度gra u0434 u0438 u0435 u043d中进行凝胶电泳 u0442 u0435（TGGE）;; 38。第35页的方法，其中动物 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0猪。; 39。 u0430 u043c u043f u043b u0438 u0444 u0438 u043a u0430 u0446 u0438 u00a0 p.35的方法，其中包括可以选择 u0430 u043c u043c u043f u043b u0438 u0444 u0438 u0446 u0438 u0440 u043e u0432 u0430 u0442 u044c第17号染色​​体的位点; 40.使用标记基因座选择第一头猪的方法，该方法用于定量指示平均日增重，根据定义，我们位于第17个染色体的第一个猪位处，距离大约70到大约72厘米，并且从遗传上来说，是 u043d u0430 u043b u0438 u0447 u0438 u00a0 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u0433 u043e与 u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u044b u043c标记选择 u0443 u043a u043a u0430 u0437 u0430 u043d u043d wow第一个包含该基因座的动物，以及用于选择与平均日增重相关的定量指标的动物; 41。标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0第40页，Dde i限制性位点的方式; 42。 p.40，Msp i限制位点的标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0的方式; 43.使用标记位点选择第一头猪的方法根据定义，对大理石的 u043c u00a0 u0441 u0430的评分评估我们在第一个猪位处 u043d u0430 u043b u0438 u0447 u0438 u00a0，位于第72号染色体处，距离约72至80 cm，遗传 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043e u043e u0433 u043e与 u043f u043e u043b u043b u0438 u043c u043e u0440 u0444 u0444 u043d u044b u043c标记选择 u0443 u043c u0430 u0437 u0430 u043d u043d哇，第一个包含该基因座的动物以及与大理石的点相关的定量指示基因的选择; 44。第43页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Msp i限制位点。; 45。第43页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Nae i限制位点; 46.使用标记位点选择第一头猪的方法，用于与该位点相关的定量指示 u0445 u0440 u0435 u0431 u0442 u043e u0432 u043e u0433 u043e的平均含量伪装为 u043d u0430 u043b u0438 u0447 u0438 u00a0在大约85至90厘米范围内的第17号染色​​体上，并通过基因 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043d u043e u0433 u043e和 u043f u043e u043b u0438 u043c u043c u043e u0440 u0444 u043d u044b u043c标记选择指南会删除包含该基因座的第一只动物，并选择与平均含量相关的定量指示基因座，以使其与 u0445 u0440 u0435 u0431 u0432 u0432 u043e u0432 u043e u0433 u043e伪装; 47。第46页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Alw ni。; 48。第46页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Bse ri。; 49。 Taa i限制位点的标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 p.46的方式; 50.使用标记位点选择第一头猪的方法，用于与 u0445 u0440 u0435 u0431 u0442 u043e u0432 u043e u0433 u043e盐猪肉的平均含量，火腿回到那里是“猎人”和//或眼石“美能达”，以 u043d u0430 u043b u0438 u0447 u0438 u00a0位于第17个染色体，位于约97至99 cm的区域内，并且在遗传上约 u0441 u0432 u00a0 u0437 u0430 u043d u043f u043e u043b u0438 u043c u043e u0440 u0444 u043d u044b u043c标记上的u043d u043e u0433，包含该基因座的第一只动物的选择以及与该基因有关的定量指示基因的选择盐猪肉的平均含量为 u0445 u0440 u0435 u0431 u0442 u043e u0432 u043e u0433 u043e火腿在那里放回“猎人”和/或“美能达火腿回到那里。” 51。转到第50页，其中标记 u 00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0 Mse i限制位点。; 52。第50页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Bstui。53。方法，用于使用标记位点选择第一头猪，以与厚度 u0445 u0440 u0435 u0431 u0442 u043e u0432 u043e u0433 u043e伪装“ Aloca ”位置p2， u043f u0440 u0435 u0434 u0443 u0441 u0431 u043c u0430 u0442 u0440 u0438 u0432 u0430 u044e u0449 u0438“位于17号染色​​体上的第一个猪基因座，从约100厘米到约103厘米，并在遗传上 u0441 u0432 u00a0 u0437 u0430 u043d u043d u043d u043e u0433 u043e与 u043f u043e u043b u0438 u043c u043c u043e u0440 u0444 u043d u044b u043c标记选择顺序 u0430 u043d u043d u043e u0433 u043e第一个包含该基因座的动物，以及与厚度 u0445 u0440 u0435 u0431 u0442 u043e u0432 u043e u0433 u043e在位置p2处伪装“ Aloca”; 54。第53页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0限制位点Alw ni。; 55。第53页，Taq i限制性酶切位点; 56. Taq i限制位点。第53页的方法，其中标记 u00a0 u0432 u043b u00a0 u0435 u0442 u0441 u00a0服务器i限制位点。

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